You have not included the most important part; the exact pw supply you are running.
Next when you change a motherboard, windows is most likely not going to boot unless you follow the procedure outlined at the top of the page for swapping a board without a clean install.
Since that procedure starts with the old motherboard still installed, your only real option is to clean install windows.
You need a working system to make the bootable usb. Use the media creation tool; Download Windows 10 (microsoft.com)
After you make the bootable usb, insert the usb into a port on the problem system. Open the side cover and pull the pw connectors from ALL drives; both ssd, hd, and optical. Have only the M.2 drive installed. Now pw on and access the boot menu. For an asus board, hit the F8 key during POST; this brings up the quick boot menu. Choose the usb. Choose custom install and delete ALL partitions on your M.2. Let setup continue. Once done, you will need to install all of your drivers, ie chipset, sound, video, wifi, whatever. Here is a link to the support page for your board; ROG STRIX B550-F GAMING (WI-FI) | ROG Strix | Gaming Motherboards|ROG - Republic of Gamers|ROG USA (asus.com)
You download all of your drivers from that site with the exception of the video driver. You need to go to the nvidia site for that driver. Make sure you install the chipset driver first then the rest of them. Reboot each time when prompted.
When you are finished with the install, you can shutdown and reconnect any drives you disconnected. It is important to do this since windows set will install wherever you want however it installs the boot files to the root of the drive on the first sata port. While this does work, you have a problem if you ever replace that drive. With no other drives installed, setup has no choice; it must install all files to the M.2.
Next when you change a motherboard, windows is most likely not going to boot unless you follow the procedure outlined at the top of the page for swapping a board without a clean install.
Since that procedure starts with the old motherboard still installed, your only real option is to clean install windows.
You need a working system to make the bootable usb. Use the media creation tool; Download Windows 10 (microsoft.com)
After you make the bootable usb, insert the usb into a port on the problem system. Open the side cover and pull the pw connectors from ALL drives; both ssd, hd, and optical. Have only the M.2 drive installed. Now pw on and access the boot menu. For an asus board, hit the F8 key during POST; this brings up the quick boot menu. Choose the usb. Choose custom install and delete ALL partitions on your M.2. Let setup continue. Once done, you will need to install all of your drivers, ie chipset, sound, video, wifi, whatever. Here is a link to the support page for your board; ROG STRIX B550-F GAMING (WI-FI) | ROG Strix | Gaming Motherboards|ROG - Republic of Gamers|ROG USA (asus.com)
You download all of your drivers from that site with the exception of the video driver. You need to go to the nvidia site for that driver. Make sure you install the chipset driver first then the rest of them. Reboot each time when prompted.
When you are finished with the install, you can shutdown and reconnect any drives you disconnected. It is important to do this since windows set will install wherever you want however it installs the boot files to the root of the drive on the first sata port. While this does work, you have a problem if you ever replace that drive. With no other drives installed, setup has no choice; it must install all files to the M.2.