So, not having anything to do that requires the heavy use of computer resources, I decide to open the application, do a manual scan of the drives, and see if it picks up anything. After it finished, I typed in a file name and it found it. I went to where the database file was supposed to be located and a new database.dbs file was there. At this point, I'm going to assume that I have to perform and complete a manual scan at least once after deleting the database.dbs file before Locate32 will create a new database.dbs file.